منابع مشابه
Understanding Accuracy in SMRT Sequencing
Introduction Single Molecule, Real-Time (SMRT) DNA sequencing achieves highly accurate sequencing results, exceeding 99.999% (Q50) accuracy, regardless of the DNA's sequence context or GC content. This is possible because SMRT Sequencing excels in all three categories that are relevant when considering accuracy in DNA sequencing: 1. Consensus accuracy 2. Sequence context bias 3. Mappability of ...
متن کاملSequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system
Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina's MiSeq, have allowed researchers to obtain millions of high quality but short sequences. The result of the added sequencing depth has been significant improv...
متن کاملWhite Paper - Detecting DNA Base Modifications Using SMRT Sequencing
Traditionally, it has been a challenge to study the wide variety of base modifications that are seen in nature. Most high-throughput techniques focus only on cytosine methylation and involve both bisulfite sequencing to convert unmethylated cytosine nucleotides to uracil nucleotides, and comparison of sequence reads from bisulfite-treated and untreated samples. SMRT sequencing, in contrast, doe...
متن کاملSMRT sequencing data for Garcinia mangostana L. variety Mesta
The "Queen of Fruits" mangosteen (Garcinia mangostana L.) produces commercially important fruits with desirable taste of flesh and pericarp rich in xanthones with medicinal properties. To date, only limited knowledge is available on the cytogenetics and genome sequences of a common variety of mangosteen (Abu Bakar et al., 2016 [1]). Here, we report the first single-molecule real-time (SMRT) seq...
متن کاملDefining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
Base J (β-D-glucosyl-hydroxymethyluracil) replaces 1% of T in the Leishmania genome and is only found in telomeric repeats (99%) and in regions where transcription starts and stops. This highly restricted distribution must be co-determined by the thymidine hydroxylases (JBP1 and JBP2) that catalyze the initial step in J synthesis. To determine the DNA sequences recognized by JBP1/2, we used SMR...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2013
ISSN: 1474-760X
DOI: 10.1186/gb-2013-14-6-405